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專利授權區
專利名稱(中)	METHOD FOR DETERMINING GLUCOSE CONCENTRATION IN THREE-DIMENSIONAL TUMOR SPHEROID IN VITRO
專利名稱(英)	METHOD FOR DETERMINING GLUCOSE CONCENTRATION IN THREE-DIMENSIONAL TUMOR SPHEROID IN VITRO
專利家族	中華民國：I919395 美國：US2026/0056129A1(公開號)
專利權人	國立清華大學 100.00%
發明人	曾繁根,戴惠美
技術領域	生化醫藥

專利摘要(中)
A method for determining glucose concentration in a three-dimensional tumor spheroid in vitro includes: adding a surface-enhanced Raman spectroscopy (SERS)-based glucose nanosensor to a co-culture of cancer cells and stromal cells so as to form a first mixture; adding the first mixture into microwells of a polydimethylsiloxane(PDMS)-based microwell array chip; subjecting the first mixture in the PDMS-based microwell array chip to incubation at 37°C so as to form the three-dimensional tumor spheroid with the SERS-based glucose nanosensor embedded therein; transferring the three-dimensional tumor spheroid onto a glass slide to allow the three-dimensional tumor spheroid to be mounted thereon; subjecting the three-dimensional tumor spheroid to confocal Raman spectroscopy so as to obtain Raman mapping images of the three-dimensional tumor spheroid; and extracting Raman intensities from the Raman mapping images and mapping the Raman intensities to a calibration curve so as to determine glucose concentration in the three-dimensional tumor spheroid in vitro

專利摘要(中)

A method for determining glucose concentration in a three-dimensional tumor spheroid in vitro includes: adding a surface-enhanced Raman spectroscopy (SERS)-based glucose nanosensor to a co-culture of cancer cells and stromal cells so as to form a first mixture; adding the first mixture into microwells of a polydimethylsiloxane(PDMS)-based microwell array chip; subjecting the first mixture in the PDMS-based microwell array chip to incubation at 37°C so as to form the three-dimensional tumor spheroid with the SERS-based glucose nanosensor embedded therein; transferring the three-dimensional tumor spheroid onto a glass slide to allow the three-dimensional tumor spheroid to be mounted thereon; subjecting the three-dimensional tumor spheroid to confocal Raman spectroscopy so as to obtain Raman mapping images of the three-dimensional tumor spheroid; and extracting Raman intensities from the Raman mapping images and mapping the Raman intensities to a calibration curve so as to determine glucose concentration in the three-dimensional tumor spheroid in vitro

專利摘要(英)
A method for determining glucose concentration in a three-dimensional tumor spheroid in vitro includes: adding a surface-enhanced Raman spectroscopy (SERS)-based glucose nanosensor to a co-culture of cancer cells and stromal cells so as to form a first mixture; adding the first mixture into microwells of a polydimethylsiloxane(PDMS)-based microwell array chip; subjecting the first mixture in the PDMS-based microwell array chip to incubation at 37°C so as to form the three-dimensional tumor spheroid with the SERS-based glucose nanosensor embedded therein; transferring the three-dimensional tumor spheroid onto a glass slide to allow the three-dimensional tumor spheroid to be mounted thereon; subjecting the three-dimensional tumor spheroid to confocal Raman spectroscopy so as to obtain Raman mapping images of the three-dimensional tumor spheroid; and extracting Raman intensities from the Raman mapping images and mapping the Raman intensities to a calibration curve so as to determine glucose concentration in the three-dimensional tumor spheroid in vitro

專利摘要(英)

聯絡資訊
承辦人姓名	楊慧敏
承辦人電話	(03)571-5131 #31181
承辦人Email	hmyang@mx.nthu.edu.tw