| A sperm sorting device including a collection chamber, a waste chamber, a sorting channel, and a material layer. The waste chamber is located on one side of the collection chamber. The sorting channel extends between the collection chamber and the waste chamber. The sorting channel is communicated with the collection chamber and the waste chamber. The sorting channel includes a lower surface, an upper surface, and a sidewall. The sidewall is located between the lower surface and the upper surface. The sidewall is connected to the lower surface and the upper surface. The material layer is located on the lower surface and the upper surface of the sorting channel. The material layer has a negative potential or a positive potential. The negative potential ranges from -1 mV to -30 mV. The positive potential ranges from 1 mV to 30 mV. |