A biomedical detection chip comprising a first detection module and a second detection module located below the first detection module. The first detection module includes a first substrate and at least one first flow path unit formed on the first substrate, each of the first flow path units including a first flow path located in the first substrate, and a first opening and a second opening located at two ends of the first flow path unit, the second detection module including a second substrate, and at least one second flow path unit corresponding in number to the first flow path unit, each of the second flow path units including a second flow path located in the second substrate, and a third opening and a fourth opening located at two ends of the second flow path, and the first substrate does not cover the fourth opening. With the design of stacking a plurality of detection modules, an effect of simultaneously detecting multiple organ cells can be achieved. |