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專利名稱(中) | 用於偵測檢體中冠狀病毒的棘蛋白受體結合域與人類第二型血管收縮素轉換酶結合的方法及平台 |
專利名稱(英) | METHOD AND PLATFORM FOR ENHANCING DETECTION ACTIVITY OF INTERACTION BETWEEN SPIKE PROTEIN RECEPTOR BINDING DOMAIN OF CORONAVIRUS FROM SPECIMEN AND HUMAN ANGIOTENSIN-CONVERTING ENZYME II |
專利家族 |
中華民國:I800853 美國:US 2022/0412959 A1(公開號) |
專利權人 | 國立清華大學 100.00% |
發明人 | 王慧菁 |
技術領域 | 生化醫藥 |
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本發明提供一種用於偵測檢體中冠狀病毒的棘蛋白受體結合域與人類第二型血管收縮素轉換酶結合的方法及平台。本發明的方法及平台採用可被切割的冷光素酶作為冠狀病毒(例如新型冠狀病毒)棘蛋白受體結合域與第二型血管收縮素轉換酶兩者結合時的報導檢測,此檢測搭配藥物篩選實行於細胞層級上,可藉由接收冷光訊號的強弱判斷藥物影響兩分子間交互作用的強度,檢測時間 可在20分鐘內完成。 |
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The present disclosure provides a method and a platform for detecting binding ofa spike protein receptor binding domain of coronavirus in a specimen with a human angiotensin-converting enzyme II. The method and the platform of the presentdisclosure use a cleavable luciferase as a report test for the combination of a spike protein receptor binding domain of coronavirus (such as novel coronavirus) andangiotensin-converting enzyme II. Screening is carried out at the cellular level. The strength of the drug’s influence on the interaction between the two molecules can bejudged by the strength of the luminescence signal. The detection time can be completedwithin 20 minutes. |
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承辦人姓名 | 劉千綺 |
承辦人電話 | 03-571-5131 #31181 |
承辦人Email | chienchi@mx.nthu.edu.tw |