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專利授權區

專利授權區
專利名稱(中)	不可逆共價鍵醣蛋白固定方法
專利名稱(英)	IRREVERSIBLE AND COVALENT METHOD FOR IMMOBILIZING GLYCOPROTEIN
專利家族	中華民國：I754939 美國：US-2021-0373003-A1(公開號)
專利權人	國立清華大學 100%
發明人	林俊成,范辰佑,陳玉如
技術領域	生化醫藥

專利摘要(英)
An irreversible and covalent method for immobilizing glycoprotein is provided, including the following steps. An organic boronic acid and a photoaffinity reagent are provided to a surface of a sloid support. The organic boronic acid is represented by R1-ArB(OH)2, wherein -ArB(OH)2 is a boronic acid group, R1 is the first linker, and the organic boronic acid is bound to the surface of the sloid support through the first linker R1. The photoaffinity reagent is bound to the surface of the sloid support through the second linker R2. After that, a glycoprotein is provided to the organic boronic acid, and the glycoprotein includes Fc fragment. The alcohol group on the sugar chain of the Fc fragment forms an organic boronate ester with the boronic acid group, so as to stabilize the glycoprotein. Next, UV light irradiation is performed, so that a covalent crosslinking is formed between the photoaffinity reagent and the glycoprotein.

專利摘要(英)

An irreversible and covalent method for immobilizing glycoprotein is provided, including the following steps. An organic boronic acid and a photoaffinity reagent are provided to a surface of a sloid support. The organic boronic acid is represented by R1-ArB(OH)2, wherein -ArB(OH)2 is a boronic acid group, R1 is the first linker, and the organic boronic acid is bound to the surface of the sloid support through the first linker R1. The photoaffinity reagent is bound to the surface of the sloid support through the second linker R2. After that, a glycoprotein is provided to the organic boronic acid, and the glycoprotein includes Fc fragment. The alcohol group on the sugar chain of the Fc fragment forms an organic boronate ester with the boronic acid group, so as to stabilize the glycoprotein. Next, UV light irradiation is performed, so that a covalent crosslinking is formed between the photoaffinity reagent and the glycoprotein.

聯絡資訊
承辦人姓名	李佳玲
承辦人電話	03-5715131 #62300
承辦人Email	cl.lee@mx.nthu.edu.tw