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專利授權區

專利授權區
專利名稱(中)	不可逆共價鍵醣蛋白固定方法
專利名稱(英)	IRREVERSIBLE AND COVALENT METHOD FOR IMMOBILIZING GLYCOPROTEIN
專利家族	中華民國：I754939 美國：US-2021-0373003-A1(公開號)
專利權人	國立清華大學 100.00%
發明人	林俊成,范辰佑,陳玉如
技術領域	生化醫藥

專利摘要(中)
本發明提供一種不可逆共價鍵醣蛋白固定方法，包括以下步驟。提供有機硼酸及光親合試劑接觸至載體表面，有機硼酸以R1-ArB(OH)2表示，-ArB(OH)2為硼酸基，R1為第一交聯劑，有機硼酸藉由第一交聯劑R1結合至載體表面，光親合試劑藉由第二交聯劑R2結合至載體表面。之後，提供醣蛋白接觸至有機硼酸，醣蛋白包括Fc片段，其中Fc片段的醣鏈上的醇基與有機硼酸的硼酸基形成有機硼酸酯，以藉此固定醣蛋白。然後，進行紫外光照射，以使光親合試劑與醣蛋白形成共價鍵交聯。

專利摘要(中)

本發明提供一種不可逆共價鍵醣蛋白固定方法，包括以下步驟。提供有機硼酸及光親合試劑接觸至載體表面，有機硼酸以R1-ArB(OH)2表示，-ArB(OH)2為硼酸基，R1為第一交聯劑，有機硼酸藉由第一交聯劑R1結合至載體表面，光親合試劑藉由第二交聯劑R2結合至載體表面。之後，提供醣蛋白接觸至有機硼酸，醣蛋白包括Fc片段，其中Fc片段的醣鏈上的醇基與有機硼酸的硼酸基形成有機硼酸酯，以藉此固定醣蛋白。然後，進行紫外光照射，以使光親合試劑與醣蛋白形成共價鍵交聯。

專利摘要(英)
An irreversible and covalent method for immobilizing glycoprotein is provided, including the following steps. An organic boronic acid and a photoaffinity reagent are provided to a surface of a sloid support. The organic boronic acid is represented by R1-ArB(OH)2, wherein -ArB(OH)2 is a boronic acid group, R1 is the first linker, and the organic boronic acid is bound to the surface of the sloid support through the first linker R1. The photoaffinity reagent is bound to the surface of the sloid support through the second linker R2. After that, a glycoprotein is provided to the organic boronic acid, and the glycoprotein includes Fc fragment. The alcohol group on the sugar chain of the Fc fragment forms an organic boronate ester with the boronic acid group, so as to stabilize the glycoprotein. Next, UV light irradiation is performed, so that a covalent crosslinking is formed between the photoaffinity reagent and the glycoprotein.

專利摘要(英)

An irreversible and covalent method for immobilizing glycoprotein is provided, including the following steps. An organic boronic acid and a photoaffinity reagent are provided to a surface of a sloid support. The organic boronic acid is represented by R1-ArB(OH)2, wherein -ArB(OH)2 is a boronic acid group, R1 is the first linker, and the organic boronic acid is bound to the surface of the sloid support through the first linker R1. The photoaffinity reagent is bound to the surface of the sloid support through the second linker R2. After that, a glycoprotein is provided to the organic boronic acid, and the glycoprotein includes Fc fragment. The alcohol group on the sugar chain of the Fc fragment forms an organic boronate ester with the boronic acid group, so as to stabilize the glycoprotein. Next, UV light irradiation is performed, so that a covalent crosslinking is formed between the photoaffinity reagent and the glycoprotein.

聯絡資訊
承辦人姓名	李馥如