|A circulating tumor cell capturing device includes a chip system and a pump. The chip system includes a confluence chip and lower chip set. The lower chip set is disposed on a lower surface of the confluence chip, and including a channel chip, a split chip, and a porous membrane. The channel chip is disposed on the lower surface of the confluence chip. The split chip is detachably disposed under the channel chip. The porous membrane is embedded disposed inside of the channel chip. The sample can flow between the channel chip and the split chip through the porous film, and the circulating tumor cell can be trap on the porous membrane. Therefore, it is favorable for enriching live circulating tumor cell or directly conducting in-situ analysis of drug resistance and staining analysis by the circulating tumor cell capturing system of the present disclosure so as to provide users with fast and accurate way of analyzing circulating tumor cell.