A manufacturing device and a manufacturing method of a test strip. The manufacturing device includes a fluid push module, a fluid flow module and a fluid output module. The fluid push module includes an actuator and a transmission unit. The transmission unit has at least a fluid delivery member. The fluid flow module includes a reagent storage unit and a reagent delivery unit. The reagent storage unit has at least one reagent storage chamber. The reagent delivery unit has at least one drain chamber, at least one first infusion line, at least a second infusion line and a plurality of check valves. The fluid output module includes a plurality of reagent outlets and a reagent output unit, and one end of each of the reagent outlets is configured with an inner recess. The reagent output unit has a plurality of channels corresponding to the reagent outlets. |