專利授權區國立清華大學國際產學營運總中心 Operations Center for Industry Collaboration

搜尋專利授權區

關鍵字

» 新增關鍵字

選單

專利授權區

專利授權區
專利名稱(中)	細胞分選方法
專利名稱(英)	CELL SORTING METHOD
專利家族	PCT：WO 2023/173239 A1(公開號) 中華民國：I825620 大陸：CN 116790497 A(公開號) 歐盟：WO 2023/173239 A1(公開號) 美國：US-2023-0287337-A1(公開號)
專利權人	國立清華大學 100.00%
發明人	曾繁根,吳仁貴,簡志軒
技術領域	光電光學

專利摘要(中)
揭露細胞分選方法。提供血液樣品。對血液樣品進行離心，以獲取含有目標細胞與非目標細胞的細胞混合物。加入螢光標記物至細胞混合物，使螢光標記物與目標細胞結合。加入水膠溶液使細胞混合物與水膠溶液混合，形成混合溶液。將混合溶液加入至陣列晶片的細胞井中，細胞混合物中的目標細胞與非目標細胞以大致單層的方式平鋪於所述細胞井的底部。使混合溶液固化。進行螢光影像分析，挑選出與螢光標記物結合後發出螢光的目標細胞，以分離所挑選的目標細胞。

專利摘要(中)

揭露細胞分選方法。提供血液樣品。對血液樣品進行離心，以獲取含有目標細胞與非目標細胞的細胞混合物。加入螢光標記物至細胞混合物，使螢光標記物與目標細胞結合。加入水膠溶液使細胞混合物與水膠溶液混合，形成混合溶液。將混合溶液加入至陣列晶片的細胞井中，細胞混合物中的目標細胞與非目標細胞以大致單層的方式平鋪於所述細胞井的底部。使混合溶液固化。進行螢光影像分析，挑選出與螢光標記物結合後發出螢光的目標細胞，以分離所挑選的目標細胞。

專利摘要(英)
Cell sorting methods are disclosed. A blood sample is provided. The blood sample is centrifuged to obtain a cell mixture having target cells and non-target cells. one or more fluorescent markers are added to the cell mixture to allow the fluorescent marker(s) to bind with the target cells. A hydrogel solution is added and mixed with the cell mixture to form a mixture solution. The mixture solution is distributed to cell wells of an array chip, and the target cells and non-target cells in the cell mixture are spread on the bottom of the cell wells in a substantially monolayer manner. The mixture solution is cured. Fluorescence image analysis is performed to select the target cells that are bound with fluorescent marker(s) and emit fluorescence, and the selected target cells are separated.

專利摘要(英)

Cell sorting methods are disclosed. A blood sample is provided. The blood sample is centrifuged to obtain a cell mixture having target cells and non-target cells. one or more fluorescent markers are added to the cell mixture to allow the fluorescent marker(s) to bind with the target cells. A hydrogel solution is added and mixed with the cell mixture to form a mixture solution. The mixture solution is distributed to cell wells of an array chip, and the target cells and non-target cells in the cell mixture are spread on the bottom of the cell wells in a substantially monolayer manner. The mixture solution is cured. Fluorescence image analysis is performed to select the target cells that are bound with fluorescent marker(s) and emit fluorescence, and the selected target cells are separated.

聯絡資訊
承辦人姓名	楊美茹
承辦人電話	03-5715131 #62305
承辦人Email	mjyang2@mx.nthu.edu.tw