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專利授權區

專利授權區
專利名稱(中)	戀臭假單胞菌基因編輯系統及其應用
專利名稱(英)	GENE EDITING SYSTEM OF PSEUDOMONAS PUTIDA AND APPLICATION THEREOF
專利家族	中華民國：I739247 美國：US-2021-0189387-A1(公開號)
專利權人	國立清華大學 62.44% ,長春人造樹脂廠股份有限公司 18.78% ,大連化學工業股份有限公司 7.04% ,長春石油化學股份有限公司 11.74%
發明人	胡育誠,范玉南,陳倬翊,周俊彥
技術領域	生化醫藥

專利摘要(英)
The invention relates to a gene editing system of Pseudomonas putida including a Pseudomonas putida, a RedCas expression plasmid and an exogenous gene expression plasmid. The RedCas expression plasmid includes a first replication origin, a first antibiotic resistance gene, a λ-Red expression cassette and a Cas expression cassette. The exogenous gene expression plasmid includes a second replication origin, a left homology arm, a second antibiotic resistance gene, an exogenous gene expression cassette, a right homology arm and a gRNA cassette. The gene editing system of P. putida includes CRISPR system and λ-Red system, which can homologously integrate of an exogenous gene into the polyploid chromosome of the P. putida to obtain a homologous recombinant with stable expression of the exogenous gene.

專利摘要(英)

The invention relates to a gene editing system of Pseudomonas putida including a Pseudomonas putida, a RedCas expression plasmid and an exogenous gene expression plasmid. The RedCas expression plasmid includes a first replication origin, a first antibiotic resistance gene, a λ-Red expression cassette and a Cas expression cassette. The exogenous gene expression plasmid includes a second replication origin, a left homology arm, a second antibiotic resistance gene, an exogenous gene expression cassette, a right homology arm and a gRNA cassette. The gene editing system of P. putida includes CRISPR system and λ-Red system, which can homologously integrate of an exogenous gene into the polyploid chromosome of the P. putida to obtain a homologous recombinant with stable expression of the exogenous gene.

聯絡資訊
承辦人姓名	李佳玲
承辦人電話	03-5715131 #62300
承辦人Email	cl.lee@mx.nthu.edu.tw