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專利名稱(中)	維斯假絲酵母的基因編輯系統、其基因編輯方法、生產十二烷二酸的轉型株及生產十二烷二酸的方法
專利名稱(英)	GENE EDITING SYSTEM OF CANDIDA VISWANATHII, GENE EDITING METHOD THEREOF, TRANSFORMANT FOR PRODUCING DODECANEDIOIC ACID, AND METOHD FOR PRODUCING DODECANEDIOIC ACID
專利家族	中華民國：I857355 美國：US-2024-0110208-A1(公開號)
專利權人	國立清華大學 58.33% ,大連化學工業股份有限公司 10.42% ,長春人造樹脂廠股份有限公司 20.83% ,長春石油化學股份有限公司 10.42%
發明人	胡育誠,范玉南,周俊彥,王興運,劉珈男
技術領域	生化醫藥

專利摘要(中)
本發明提供一種維斯假絲酵母的基因編輯系統，包含維斯假絲酵母、第一基因編輯片段以及第二基因編輯片段。第一基因編輯片段包含第一同源臂及篩選基因。第二基因編輯片段包含第二同源臂、Cas9表現卡匣及sgRNA卡匣。Cas9表現卡匣包含Cas9啟動子、Cas9基因和三核定位序列。sgRNA卡匣包含sgRNA啟動子、第一核酶、靶向序列、支架序列和第二核酶。第一基因編輯片段與第二基因編輯片段構成一線性片段，用以對維斯假絲酵母之染色體進行基因編輯。藉此可便捷地編輯維斯假絲酵母的每套目標染色體，並能在無任何特定條件下得到穩定的轉型株。

專利摘要(中)

本發明提供一種維斯假絲酵母的基因編輯系統，包含維斯假絲酵母、第一基因編輯片段以及第二基因編輯片段。第一基因編輯片段包含第一同源臂及篩選基因。第二基因編輯片段包含第二同源臂、Cas9表現卡匣及sgRNA卡匣。Cas9表現卡匣包含Cas9啟動子、Cas9基因和三核定位序列。sgRNA卡匣包含sgRNA啟動子、第一核酶、靶向序列、支架序列和第二核酶。第一基因編輯片段與第二基因編輯片段構成一線性片段，用以對維斯假絲酵母之染色體進行基因編輯。藉此可便捷地編輯維斯假絲酵母的每套目標染色體，並能在無任何特定條件下得到穩定的轉型株。

專利摘要(英)
The invention relates to a gene editing system of Candida viswanathii including a Candida viswanathii, a first gene editing fragment, and a second gene editing fragment. The first gene editing fragment includes a first homology arm and a first selection gene fragment. The second gene editing fragment includes a second homology arm, a Cas9 expression cassette, and a sgRNA cassette. The Cas9 expression cassette includes a Cas9 promoter, a Cas9 gene, and three nuclear localization signals. The sgRNA cassette includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold sequence, and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment so as to perform gene editing on a chromosome of the Candida viswanathii. Therefore, every target chromosome of the Candida viswanathii can be edited easily so as to attain a stable recombinant without any specific condition.

專利摘要(英)

The invention relates to a gene editing system of Candida viswanathii including a Candida viswanathii, a first gene editing fragment, and a second gene editing fragment. The first gene editing fragment includes a first homology arm and a first selection gene fragment. The second gene editing fragment includes a second homology arm, a Cas9 expression cassette, and a sgRNA cassette. The Cas9 expression cassette includes a Cas9 promoter, a Cas9 gene, and three nuclear localization signals. The sgRNA cassette includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold sequence, and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment so as to perform gene editing on a chromosome of the Candida viswanathii. Therefore, every target chromosome of the Candida viswanathii can be edited easily so as to attain a stable recombinant without any specific condition.

聯絡資訊
承辦人姓名	李馥如