A chip for sample separation including a first substrate, a first electrode, a first dielectric layer, a second substrate, a second electrode, a second dielectric layer, and a flow channel layer is provided. The first electrode is disposed on the first substrate. The first dielectric layer is disposed on the first electrode and includes a first opening. The first opening exposes a portion of the first electrode. The second electrode is disposed on the second substrate. The second dielectric layer is disposed on the second electrode and includes a second opening. The second opening exposes a portion of the second electrode. An area of the first electrode exposed by the first opening is smaller than an area of the second electrode exposed by the second opening. The flow channel layer is sandwiched between the first dielectric layer and the second dielectric layer and includes a through hole. The through hole communicates between the first opening and the second opening. |