A sperm sorting apparatus and a sperm sorting method are provided. The apparatus includes a medium chamber, a waste chamber and a sorting channel. The
medium chamber is configured to accommodate a medium solution. The waste chamber is disposed aside the medium chamber, and configured to accommodate a
waste solution obtained after sorting and including low motility or dead sperms. The sorting channel, which configured to receive a sperm sample, extends along a first direction between the chambers, and communicates with the chambers. Sperms in the sperm sample react in corresponding to a medium solution flow from the medium chamber, so as to be sorted. The sorting channel has a first portion and a second portion. A first portion of the sorting channel is closer to the medium chamber than a second portion of the sorting channel. A width of the first portion along a second direction is greater than a critical dimension rangi ng from 200 μm to 400 μm. |