A fabricating method of a non-enzyme sensor element includes a printing step, a coating step and an electroplating step. In the printing step, a conductive material is printed on a surface of a substrate to form a working electrode, a reference electrode and an auxiliary electrode, and a porous carbon material is printed on the working electrode to form a porous carbon layer. In the coating step, a graphene film material is coated on the porous carbon layer to form a graphene layer. In the electroplating step, a metal is electroplated on the graphene layer by a pulse constant current and to from a catalyst layer, and the catalyst layer includes a metal oxide. Therefore, the evenly distributed and fine surface of the working electrode can be obtained, so as to increase the sensitivity and concentration sensing range of the non-enzyme sensor element. |