The present invention provides a micropore microfluidic chip system and use thereof for continuous dynamic measuring electrical properties of individual bacterium. The micropore microfluidic chip system comprises: a body having a tunnel electrode, an upper flow channel and a lower flow channel, and the body forms a micropore structure, which is the upper flow channel and the lower flow channel, wherein the tunnel electrode is formed in the lower flow channel. The present invention further provides a micropore microfluidic chip system comprising a recognition layer with imprinted polymer. The micropore microfluidic chip system of the present invention can measure the real-time electrical properties of a single bacterium. |